Development and validation of a competitive ELISA based on virus-like particles of serotype Senecavirus A to detect serum antibodies

Abstract Virus-like particles (VLPs) are high-priority antigens with highly ordered repetitive structures, which similar to natural viral particles. We have developed a competitive enzyme-linked immunosorbent assay (cELISA) for detecting antibodies directed against Senecavirus A (SVA). Our utilizes SVA VLPs that were expressed and assembled in an E. coli expression system as the coating antigens. better safety immunogenicity than intact or peptides. The VLPs-based cELISA was used test 342 serum samples collected from different pig farms, results showed its specificity sensitivity 100% 94%, respectively. consistency rates of BIOSTONE AsurDx™ (SVA) Antibody Test Kit indirect immunofluorescent 90.0% 94.2%, Therefore, this can be effectively reliably detection discrimination infection samples.